Rapid detection of γT cell receptor gene rearrangements in acute lymphoblastic leukemia by electrophoresis and silver staining: Implications for detection of minimal residual disease

✍ Scribed by Angelo Valetto; Marina Lanciotti; Dr. Daniela Di Martino; Giorgia Anselmi; Federico Bottini; Piergiorgio Mori; Giovanni Candiano

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 321 KB
• Volume: 19
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150190831

✦ Synopsis

Rapid detection of yT cell receptor gene rearrangements in acute lymphoblastic leukemia by electrophoresis and silver staining: Implications for detection of minimal residual disease

Minimal residual disease (MRD) in acute lymphoblastic leukemia (ALL) was studied using polymerase chain reaction (PCR). yT cell receptor (TCRG) genes are ideal targets for PCR-based detection of MRD due to their molecular characteristics. Polyacrylamide gel electrophoresis (PAGE) analysis of PCR products followed by silver staining was performed for 72 children with ALL at the onset of disease. Silver staining is an effective technique to detect gene rearrangements without the use of ethidium bromide. Moreover, this method may show heteroduplex bands of a clonal nature when both TCRG alleles are rearranged. PCR products subjected to a rapid staining protocol were recovered from the gel, reamplified by a second PCR and directly sequenced. After sequencing, we identified the junctional region and obtained patient-specific probes. In more than half of the patients we detected TCRG rearrangements that were used as molecular markers for residual disease.