Rapid detection of the alternativeN-glycosylation pathway using high pH anion exchange chromatography
โ Scribed by C. Villers; Y. Plancke; R. Cacan; A. Verbert
- Publisher
- Springer US
- Year
- 1994
- Tongue
- English
- Weight
- 428 KB
- Volume
- 11
- Category
- Article
- ISSN
- 1573-4986
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Fucosylated oligosaccharides of the /3Gal(l+4)GlcNAc-, PGal(l+4)Glc-, and /3Gal(l+3)GlcNAc+eries were chromatographed on a high-performance anion-exchange pellicular resin under alkaline conditions (pH ~13). Fucosylation of either lactose, lactosamine (Type II chains), or lacto-N-biose (Type I chain
An assay has been developed to quantitate the amount of mannose 6-phosphate in glycoproteins using high-pH anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). The method was tested on a recombinant lysosomal enzyme, human alpha-galactosidase A, that contains mannose 6-phosp