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Rapid detection of plant RNA viruses by dot blot hybridization

โœ Scribed by Stephen J. Garger; Thomas Turpen; James C. Carrington; T. Jack Morris; Ramon L. Jordan; J. Allan Dodds; Laurence K. Grill

Book ID
112824926
Publisher
Springer
Year
1983
Tongue
English
Weight
490 KB
Volume
1
Category
Article
ISSN
0735-9640

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A rapid, microanalytical procedure for the reproducible isolation of RNA from small cultured cell samples and application to dot-blot hybridization is described. The procedure employs guanidine hydrochloride solubilization of whole cells, disruption by syringing, and selective precipitation of RNA w