## Abstract Since the amount of hepatitis C virus (HCV) RNA might be correlated with the degree of severity of hepatitis and response to treatment, quantitation of HCV RNA in serum was established using competitive polymerase chain reaction (PCR). Known amounts of a plasmid containing HCVβcDNA were
Quantitative detection of hepatitis C virus RNA by polymerase chain reaction and dot blot hybridization
β Scribed by KOBAYASHI, Y; WATANABE, S; KONISHI, M; YOKOI, M; KAKEHASHI, R; KAITO, M; SUZUKI, S
- Book ID
- 123059704
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 315 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0928-4346
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## Abstract The mean prevalence of antiβhepatitis C virus (HCV) in Italy is 0.87%. It reaches 2% in Campania, Southern Italy. Approximately 50% of community acquired nonβA, nonβB (NANB) hepatitis cannot be associated with known parenteral exposure. A recent Italian study has shown that the only dem
Although detection of hepatitis C virus RNA with polymerase chain reaction has become the standard for diagnosis, extensive application has been thwarted by polymerase chain reaction's labor intensiveness, risk of false-positive results through contamination and time required for individual assays.