## Abstract The mean prevalence of anti‐hepatitis C virus (HCV) in Italy is 0.87%. It reaches 2% in Campania, Southern Italy. Approximately 50% of community acquired non‐A, non‐B (NANB) hepatitis cannot be associated with known parenteral exposure. A recent Italian study has shown that the only dem
Detection of hepatitis C virus RNA by “hot start” polymerase chain reaction
✍ Scribed by Anthony R. Carroll; Eithne O'Sullivan
- Book ID
- 118566375
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 229 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0168-8278
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Although detection of hepatitis C virus RNA with polymerase chain reaction has become the standard for diagnosis, extensive application has been thwarted by polymerase chain reaction's labor intensiveness, risk of false-positive results through contamination and time required for individual assays.
## Abstract Since the amount of hepatitis C virus (HCV) RNA might be correlated with the degree of severity of hepatitis and response to treatment, quantitation of HCV RNA in serum was established using competitive polymerase chain reaction (PCR). Known amounts of a plasmid containing HCV‐cDNA were
The role of hepatitis C virus (HCV) infection in fulminant hepatic failure is controversial. The frequency of serum HCV RNA positivity in previously reported patients with fulminant hepatic failure (FHF) of indeterminate cause ranged from 0 to 12% in the United States and Europe and from 43% to 59%