Rapid approach to the quantitative determination of Topiramate (2,3:4,5-bis-O-(1-methylethylidene)-β-D-fructopyranose sulfamate) in human plasma by liquid–liquid extraction and flow-injection negative-ion electrospray mass spectrometry

✍ Scribed by Su Chen; Paul M. Carvey

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 71 KB
• Volume: 13
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19991030)13:20<1980::aid-rcm741>3.0.co;2-c

✦ Synopsis

Topiramate, a sulfamate-substituted monosaccharide (2,3:4,5-bis-O-(1-methylethylidene)-b-D-fructopyranose sulfamate), is a new antiepileptic drug, which has been approved for adjunctive therapy in adult patients with partial-onset seizures. Liquid-liquid extraction followed by flow-injection negative-ion electrospray mass spectrometry was evaluated as a means for the quantitative analysis of Topiramate in human plasma. Prednisone (1,4-pregnadiene-17-a,21-diol-3,11,20-trione [15 mg/mL]) was used as the internal standard because its solubility and molecular weight are similar to those of Topiramate. Calibration curves for Topiramate were linear over a range of 1 to 30 mg/mL plasma (signal-to-noise ratio b4) and were highly reliable (r 2 = 0.994). This approach offers several advantages: (i) the extraction of Topiramate from human plasma using chloroform is simple and reproducible; (ii) the quantitative determination of Topiramate, in the presence of an internal standard, by flow-injection negative-ion electrospray mass spectrometry with selected-ion recording, is rapid and accurate and does not require chromatographic separation; (iii) the assay possesses adequate sensitivity (2-25 mg/mL) for the quantitative analysis of Topiramate in plasma from patients.