In vivo microdialysis and liquid chromatography/thermospray mass spectrometry of the novel anticonvulsant 2,3:4,5-bis-o-(1-methylethylidene)-β-D-fructopyranose sulfamate (topiramate) in rat brain fluid

✍ Scribed by Masucci, John A.; Ortegon, Marta E.; Jones, William J.; Shank, Richard P.; Caldwell, Gary W.

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 309 KB
• Volume: 33
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/(sici)1096-9888(199801)33:1<85::aid-jms612>3.0.co;2-r

✦ Synopsis

The concentration of a novel anticonvulsant, 2,3 : 4,5-bis-O-(1-methylethylidene)-b-D-fructopyranose sulfamate (topiramate), was determined in the extracellular Ñuid of rat brain by in vivo microdialysis combined o †-line with liquid chromatography/thermospray mass spectrometry. A microdialysis probe was stereotaxically implanted in the nucleus accumbens region of the rat brain. The maximum concentration of topiramate in the brain dialysate for a dose of 50 mg kg-1 i.v. was ¿10 lM and occurred 45 min post-injection. The detection limit of topiramate in the extracellular Ñuid of rat brain was in the 0.1 lM range using selected ion monitoring techniques. The base peak, which was the ammonium adduct ion was used for detection. An internal standard of [ M + NH 4 ] ', d 12 -labeled topiramate was utilized for quantitation by isotope dilution analysis.