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Rapid and Sensitive Method for Citrinin Determination Using High-Performance Liquid Chromatography with Fluorescence Detection

✍ Scribed by Dohnal, Vlastimil; Pavlíková, Lucie; Kuča, Kamil


Book ID
121078346
Publisher
Taylor and Francis Group
Year
2010
Tongue
English
Weight
165 KB
Volume
43
Category
Article
ISSN
0003-2719

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Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co