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Rapid and Sensitive Determination of Phenol in Honey by High-Performance Liquid Chromatography with Fluorescence Detection

✍ Scribed by Győrik, Márta; Herpai, Zoltán; Szécsényi, Ildikó; Varga, László; Szigeti, Jenő

Book ID
127107427
Publisher
American Chemical Society
Year
2003
Tongue
English
Weight
79 KB
Volume
51
Category
Article
ISSN
0021-8561

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Sialic acids were specifically labeled with o-phenylenediamine-2HCl (OPD) to yield stable fluorescent quinoxaline derivatives. The sialic acids were released from the glycoprotein in a NaHSO4 solution (0.25 M 80 degrees C, 20 min) and derivatized in the same solution with the OPD (10 mg/ml (final co