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Rapid analytical gel chromatography of proteins and peptides on Sephadex microbore columns

✍ Scribed by N. Catsimpoolas; J. Kenney

Book ID: 104144246
Publisher: Elsevier Science
Year: 1972
Tongue: English
Weight: 562 KB
Volume: 64
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)92950-9

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✦ Synopsis

A method has been developed for the rapid analytical gel chromatography of low-molecular-weight proteins and peptides on microbore columns (3 mm I.D.) packed with Sephadex G-50 and G-25. The time of a complete chromatographic analysis can be as short as 30 min using anly 15-30 pg of protein. The elution parameters of proteins are compared in terms of '?etentibn time" rather than elution volume under conditions of constant flow rate of eluent. The technique has been used successfully for the estimation of the molecular weight of peptides and proteins in the range of 1000 to 26,000 daltons. .

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