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Column chromatography on polyethylenimine-silica: Rapid resolution of nucleotides and proteins with short columns and low pressures

✍ Scribed by K. Watanabe; W.-S. Chow; G.P. Royer

Book ID
102627453
Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
314 KB
Volume
127
Category
Article
ISSN
0003-2697

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✦ Synopsis

Silica gel (100-200 mesh) can be coated with a stable layer of crosslinked polyethylenimine (PEI). The resulting material is useful in column chromatography for quantitative separation of adenine nucleotides. For example, 3':5'-cyclic AMP can be separated from a mixture containing AMP, ADP, and ATP. A mixture of adenosine, AMP, ADP, and ATP can be resolved with quantitative recovery of components. Convenient separation of cytochrome c from albumin illustrates the applicability of this system to protein purification. PEI-Xama silica gel and PEI-glutaraldehyde silica gel have ion-exchange capacities of 0.27 and 0.21 meq/g, respectively. The materials are dimensionally rigid and chemically stable except in alkaline solutions (>pH 10) for prolonged periods.

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Rapid, low pressure, and simultaneous ion chromatography of common inorganic anions and cations on short permanently coated monolithic columns
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## Abstract Short permanently coated reversed‐phase silica based monolithic columns have been investigated for the rapid separation of inorganic anions and cations. One 2.5Γ—0.46 cm column was permanently coated with didodecyldimethylammonium (DDAB), for anion analysis; and a second 5.0Γ—0.46 cm colu