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Rapid analysis of steroidal saponin mixture using electrospray ionization mass spectrometry combined with sequential tandem mass spectrometry

✍ Scribed by Shiping Fang; Chunyan Hao; Weixing Sun; Zhiqiang Liu; Shuying Liu

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 149 KB
Volume: 12
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19980529)12:10<589::aid-rcm200>3.0.co;2-q

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✦ Synopsis

Using electrospray ionization mass spectrometry (MS) combined with sequential tandem MS(ESI-MS n ), two major steroidal saponins extracted from Tribulus terrestris were studied, and considerable useful structural information was obtained. The structure of the proposed known steroidal saponin was verified, and the structure of the unknown saponin was investigated using MS n experiments. Some special fragment ions were also observed, and the corresponding fragmentation mechanisms were investigated which are characteristic for steroidal saponins and can give some information on the linkage position of some sugar groups in saponins. This methodology has been established as a powerful tool for the rapid, comparative analysis of mixtures such as crude plant extracts.

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