We describe here a general and simple procedure for cDNA library construction making use of in vitro amplification of cDNA by polymerase chain reaction (PCR). The first-strand cDNA is synthesized from total RNA with a primer EcoRI-(dT)17 and oligo(dG) tailed. An oligonucleotide, EcoRI-BamHI-(dC)13,
Rapid amplification of complementary DNA from small amounts of unfractionated RNA
โ Scribed by Paul J. Doherty; Mario Huesca-Contreras; H.Michael Dosch; Sueihua Pan
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 767 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0003-2697
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