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Rapid, accurate and sensitive determination of molecular weight of15N and13C labeled recombinant proteins by electrospray ionization mass spectrometry

✍ Scribed by Timothy G. Heath; Venkataraman Thanabal; Qi-Zhuang Ye

Publisher
Springer-Verlag
Year
1993
Tongue
English
Weight
348 KB
Volume
7
Category
Article
ISSN
0951-208X

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✦ Synopsis

Electrospray ionization mass spectrometry (ES-MS) was utilized to accurately measure the molecular weight of uniformly l5N and 13C/15N isotope labeled stromelysin catalytic domain (20 kDa), which in turn, was used to determine the degree of isotope enrichment in the protein.

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## Abstract The ring opening of 1,3‐di‐__tert__‐butylaziridinone by __tert__‐butylamine and aniline was investigated by using electrospray ionization and collision‐induced dissociation in an ion trap mass spectrometer in conjunction with ^15^N labeling of the two amine nucleophiles. Using the MS^__