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Raman microspectroscopy: a non-destructive approach for the analysis of DNA in living sperm

✍ Scribed by Sanchez, V.; Wistuba, J.; Kliesch, S.; Schlatt, S.; Mallidis, C.


Book ID
122093670
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
45 KB
Volume
100
Category
Article
ISSN
1556-5653

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## Abstract We present a simple approach that avoids the problem of coupling fluid‐sample interaction in confocal Raman depth‐profiling with immersion objectives. We use a thin protective medium (PE film) that preserves the sample from direct contact with the coupling fluid, and that can be readily