## Synopsis Salt-promoted adsorption of proteins occurs on hydrophobic gels, on immobilized metal ions e.g., Znz+, C g + , Ni2+, Cu2+, and on newly described absorbents provisionally called "thiophilic gels." The latter gels are characterized by the ligand structure -SO,-CH,-CH,-S-R. In a simple
Radiotracer techniques for protein adsorption measurements
β Scribed by Grant, Warren H. ;Smith, Leslie E. ;Stromberg, Robert R.
- Publisher
- John Wiley and Sons
- Year
- 1977
- Tongue
- English
- Weight
- 293 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0021-9304
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β¦ Synopsis
Abstract
Factors which contribute to measurement errors associated with the use of radiotracers to measure protein adsorption are considered. Techniques for removal of excess adsorbent solution and for estimation of surface area are described. Artifacts induced by the incorporation of a radioβlabel both by specific adsorption of the labeling atom and by changes in the protein are discussed.
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