A novel technique for studying the adsorption of plasma proteins on hydrophobic surfaces

✍ Scribed by Bagnall, R. D. ;Annis, J. A. D. ;Arundel, P. A.

• Publisher: John Wiley and Sons
• Year: 1978
• Tongue: English
• Weight: 444 KB
• Volume: 12
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820120507

✦ Synopsis

Abstract

A method is shown for deducing the surface area of a pendant drop from the same profile photograph as is normally used to determine interfacial tension. Manipulation of such drops by a micrometer syringe then enables the pendant drop to be used as a surface balance for studying adsorption from bulk solutions. Results are given for the compression of films from solutions of albumin, γ‐globulin, fibrinogen, albumin–γ‐globulin mixture and albumin–γ‐globulin–fibrinogen mixture, and from serum, plasma, and blood at the isooctane–buffer interface. It is suggested that γ‐globulin and fibrinogen films are unfolded at the interface but that albumin films are different in that they consist of an inner, unfolded layer and an outer, globular layer. A film from serum resembles that from γ‐globulin alone, and not that from albumin–γ‐globulin mixture, whereas a film from plasma resembles that of fibrinogen at low compression and that of albumin–γ‐globulin–fibrinogen mixture at high compression. A film from blood is shown to resemble that from plasma.