Radiolabeling of proteins by reductive a
Radiolabeling of proteins by reductive alkylation with [14C]formaldehyde and sodium cyanoborohydride
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Diane Dottavio-Martin; Joanne M. Ravel
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Article
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1978
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Elsevier Science
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English
โ 244 KB
A procedure is described for radiolabeling proteins in vitro by reductive alkylation. The proteins are treated with [W]formaldehyde in the presence of sodium cyanoborohydride, a reducing agent that is stable in aqueous solution at pH 7. The advantage of this procedure is that the reaction can be car