✦ LIBER ✦

Radioimmunological assay of prostacyclin synthetase activity

✍ Scribed by Chen L. Tai; Hsin-Hsiung Tai

Publisher: Elsevier Science
Year: 1980
Weight: 602 KB
Volume: 4
Category: Article
ISSN: 0161-4630
DOI: 10.1016/0161-4630(80)90048-8

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Lactose synthetase assay: Uridine tripho

Lactose synthetase assay: Uridine triphosphate inhibition of interfering hydrolase activity

✍ William L. McGuire 📂 Article 📅 1969 🏛 Elsevier Science 🌐 English ⚖ 407 KB

A fluorimetric assay for acyl-CoA synthe

A fluorimetric assay for acyl-CoA synthetase activities

✍ Wessel Lageweg; Ivar Steen; Joseph M. Tager; Ronald J.A. Wanders 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 415 KB

In this paper we describe a fluorimetric assay for the measurement of long-chain acyl-CoA synthetase activity in rat liver postnuclear supernatants. The method is based upon the use of acyl-CoA oxidase which catalyzes the dehydrogenation of acyl-CoA esters to yield enoyl-CoA esters and H2O2. H2O2 su

A semiautomated assay procedure for the

A semiautomated assay procedure for the determination of aminoacyl-tRNA synthetase activity

✍ Sylvia A. McCune; P.L. Yu; Walter E. Nance 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 301 KB

A semiautomated assay procedure for the determination of aminoacyl-tFWA synthetase activity is described. The assay system employs a Technicon AutoAnalyzer with a continuous filter paper attachment. A larger number of samples can be measured with greater accuracy, reproducibility, and more rapidly t

Purification of prostaglandin H syntheta

Purification of prostaglandin H synthetase and a fluorometric assay for its activity

✍ Alevtina T. Mevkh; Galina F. Sud'ina; Nikolay B. Golub; Sergey D. Varfolomeev 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 535 KB

Prostaglandin H synthetase (PGH synthetase) has been purified to homogeneity from sheep vesicular glands. The pure enzyme has a specific activity of about 40 @.I of arachidonic acid consumed per minute per milligram of protein, which corresponds to a turnover number of 2800 mitt-' per subunit. The p

Application of girard reagent T to radio

Application of girard reagent T to radioimmunological assay of prednisolone in plasma

✍ Jack C. K. Loo; Arnost B. Vilim; Nicole Jordan; James Moffatt 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 202 KB

method (pKa = 2.54) at the analytical wavelength, h 316 nm (4). At the time of preparation of this manuscript, it was found3 that the reported pKa value of methaqualone in the Schulman et al. ( 5) study was 2.59 f 0.006 by absorptiometric and fluorometric pH titration. This result is additional evid

On the Assay of Acetyl-CoA Synthetase Ac

On the Assay of Acetyl-CoA Synthetase Activity in Chloroplasts and Leaf Extracts

✍ P.G. Roughan; J.B. Ohlrogge 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 488 KB

Acetyl-CoA synthetase activity in vitro is assayed quickly and conveniently by incubating whole chloroplasts, chloroplast extracts, or leaf extracts with labeled acetate, CoA, ATP, and Mg and transferring aliquots of the reaction mixture to pieces of either Whatman No. 1 or DE81 filter paper. Unreac