Radioimmunoassay of rheumatoid factor
β Scribed by By P. Franchimont; S. Suteanu
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 523 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0004-3591
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## Abstract In this report, solid phase radioimmunoassays that specifically measure IgG rheumatoid factor (RF) and IgM RF in absolute concentrations are described. Polyclonal RF preparations were utilized as standards, and as little as 40 ng/ml of IgG RF and 1 ng/ml of IgM RF were detected. The IgG
The reactions between rheumatoid factor and erythrocytes sensitized by antibodies of human, rabbit and guinea pig origin were studied using absorption, elution, dispersion, inhibition and mixed aggIutination experiments. The results indicated that there are separate molecules of the rheumatoid facto
## Abstract A method for the separation of rheumatoid factors into two serologically distinct macroglobulins is described. Macroglobulins, reacting with cells coated with human as well as with rabbit gamma globulin, were found to be mainly 19.4S, but occasionally such preparations contained minor c