β Scribed by Ralph Heimer; Edith R. Schwartz
No coin nor oath required. For personal study only.
A method for the separation of rheumatoid factors into two serologically distinct macroglobulins is described. Macroglobulins, reacting with cells coated with human as well as with rabbit gamma globulin, were found to be mainly 19.4S, but occasionally such preparations contained minor constituents sedimenting at 22s as well as 27s. Macroglobulins which reacted with cells coated with human gamma globulin, but which were inactive when rabbit gamma globulin was used, were mainly 19.5S. Some sera, however, contained substances serologically similar in behavior but sedimenting at 17.5S, 22.0S, and 27s.
π SIMILAR VOLUMES
The reactions between rheumatoid factor and erythrocytes sensitized by antibodies of human, rabbit and guinea pig origin were studied using absorption, elution, dispersion, inhibition and mixed aggIutination experiments. The results indicated that there are separate molecules of the rheumatoid facto