## Abstract Much controversy regarding the relationship between nutrients and serum in regulation of cell growth can be reconciled by recognizing that serum contains multiple factors which regulate different events in the cell cycle. Serum was fractioned into a plateletβderived growth factor (PDGF)
Radioimmunoassay of a Human Serum Growth Factor for Balb/c-3t3 Cells: Derivation from Platelets
β Scribed by Harry N. Antoniades and Charles D. Scher
- Book ID
- 123654509
- Publisher
- National Academy of Sciences
- Year
- 1977
- Tongue
- English
- Weight
- 1000 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0027-8424
- DOI
- 10.2307/67148
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## Abstract Plateletβderived growth factor (PDGF) and plateletβpoor plasma, which lacks PDGF, both induce a rapid increase in the rate of total protein synthesis within quiescent, densityβarrested Balb/cβ3T3 cells. This stimulation of protein synthesis is associated with an increased aggregation of
Platelet-derived growth factor (PDGF) and other agents that activate protein kinase C (PKC) rapidly alter cytosolic pH (pHJ and intracellular free calcium (Ca' +I,) in BALBk-3T3 fibroblasts. To define whether changes in pH, or [Ca++], are linked to PDGF-stimulated mitogenesis, these parameters were
Recently we found that an intracellular event related to phosphatidylinositol 4,5bisphosphate (PIP2) is crucial for platelet-derived growth factor (PDGF)-induced mitogenesis in fibroblastic cells (Matuoka, K., et al.: Science 239:640-643, 1988). In the present study we examined the mitogenic effects