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Radioimmunoassay of 5-methyl-2′-deoxycytidine. A method for the quantitation of DNA methylation

✍ Scribed by J.A. Vilpo; S. Rasi; L.M. Vilpo


Book ID
118899341
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
253 KB
Volume
75
Category
Article
ISSN
0022-1759

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A radioimmunoassay (RIA) capable of quantitating dCTP in femtomolar amounts in cell extracts has been developed, and applied to human fibroblast cell lines and L5178Y mouse lymphoma lines. Cross reactivity of the antibody with CTP, though low (2.7%) has necessitated pre-RIA removal of CTP by either