Radiobiological characterization of two human chemotherapy-resistant intermediate grade non-Hodgkin's lymphoma cell lines

✍ Scribed by Amr Aref; Razmi Mohammad; Mark Yudelev; Rajani Choudhuri; Amy Strowbridge; Colin Orton; Ayad Al-Katib

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 193 KB
• Volume: 7
• Category: Article
• ISSN: 1065-7541
• DOI: 10.1002/(sici)1520-6823(1999)7:3<158::aid-roi4>3.0.co;2-b

✦ Synopsis

Intermediate grade non-Hodgkin's lymphoma (IGNHL) is generally considered a radiosensitive tumor that can be controlled with moderate radiation doses. Cell-survival curves of cell lines derived from IGNHL have been typically described to exhibit small or no shoulder, implying inability to accumulate or repair sublethal radiation damage. We characterize in this report the clonogenic radiation survival curves of two human IGNHL cell lines, WSU-DLCL 2 and SK-DHL 2 B, established from patients who expired after having exhibited chemotherapy resistance of their tumors. The cells were irradiated with 60 Co radiation at a dose rate of 85-100 cGy/min and cell survival data were analyzed according to the linear quadratic model. The ␣/␤ values for WSU-DLCL 2 and SK-DHL 2 B cells are 2 and 8.6, respectively. The corresponding SF 2 are 0.42 and 0.35, respectively. Both cell lines are able to repair radiation-induced sublethal damage. These data indicate that these cells are only moderately radiosensitive.