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Radiation effects of thymidine-3H and thymidine-14C

✍ Scribed by H.A. McQuade; M. Friedkin

Book ID
118874105
Publisher
Elsevier Science
Year
1960
Tongue
English
Weight
502 KB
Volume
21
Category
Article
ISSN
0014-4827

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πŸ“œ SIMILAR VOLUMES

Radiolysis of 14C- and 3H-labelled thymi
Radiolysis of 14C- and 3H-labelled thymidine
✍ J. KopoldovΓ‘; V. DedkΗ’vΓ‘ πŸ“‚ Article πŸ“… 1975 πŸ› John Wiley and Sons 🌐 French βš– 519 KB

## Abstract 10^βˆ’3^M oxygenated aqueous solutions of thymidine preparations, labelled with ^14^C and ^3^H in various positions were gammairradiated. Radiation produats formed were followed in dependenae of irradiation doses. Their yields were confronted with produats formed during the autoradiolysis

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## Abstract We report a three‐step synthesis of thymidine, starting from trace amounts of urea, that allows rapid introduction of radioactivity in C2‐position of the pyrimidine ring. Thymine was produced within 5 min with a 60% yield by cyclocondensation of urea and diethyl β‐methylmalate in fuming

Isotopic discrimination between 3H and 1
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We compare the phosphorylation of 6-3H-TdR and 2-14C-TdR to TMP, TDP and TTP by tissue extracts and to T M P by purified kiizase pre~aratioiz~. The rate of'pho~phory~ation of'& 3H-TdR is only 0.6 to 0.8 that of 2-14C-TdR. The same isotopic discrimination exists between methyl-labelled thymidines. In