Rabbit bone marrow glucose-6-phosphate dehydrogenase during erythroid cell development

✍ Scribed by Paolino Ninfalil; Fulvio Palma; Giorgio Fornaini

• Book ID: 104673839
• Publisher: Springer
• Year: 1987
• Tongue: English
• Weight: 561 KB
• Volume: 75
• Category: Article
• ISSN: 0300-8177
• DOI: 10.1007/bf00229896

✦ Synopsis

Studies were carried out on glucose-6-phosphate dehydrogenase (G6P-DH) during the differentiation of rabbit bone marrow erythroid cells. It was found that G6P-DH, although displaying a 7-fold activity decrease, did not change the relative amounts of its three dimeric forms. Using homogeneous enzyme preparations, we observed that from dividing to non-dividing erythroblasts the following properties remained constant: V max dependence on pH and temperature, Km for G6P dependence on pH, heat stability, 2-deoxy glucose-6-phosphate utilization, molecular weight, while the Km for NADP significantly increased in non-dividing erythroblasts. These results indicate that no shift towards the oxidized form of the enzyme and no substantial modifications of the protein take place during cell differentiation.