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R-banding and nonisotopic in situ hybridization: precise localization of the human type II collagen gene (COL2A1)

✍ Scribed by Ei-ichi Takahashi; Tada-aki Hori; Peter O'Connell; Mark Leppert; Ray White

Publisher
Springer
Year
1990
Tongue
English
Weight
714 KB
Volume
86
Category
Article
ISSN
0340-6717

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✦ Synopsis

A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q13.11-q13.12 in this manner, to illustrate the potential of the technique for improving the precision of chromosomal mapping and physical ordering of genes.

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