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Quercetin modulates activities of Taiwan cobra phospholipase A2via its effects on membrane structure and membrane-bound mode of phospholipase A2

✍ Scribed by Chiou, Yi-Ling; Lin, Shinne-Ren; Hu, Wan-Ping; Chang, Long-Sen

Book ID
113094051
Publisher
Indian Academy of Sciences
Year
2012
Tongue
English
Weight
434 KB
Volume
37
Category
Article
ISSN
0250-5991

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πŸ“œ SIMILAR VOLUMES

Mutations on N-terminal region of Taiwan
Mutations on N-terminal region of Taiwan cobra phospholipase A2 result in structurally distorted effects
✍ Yi-Ling Chiou; Shinne-Ren Lin; Long-Sen Chang πŸ“‚ Article πŸ“… 2008 πŸ› John Wiley and Sons 🌐 English βš– 315 KB

## Abstract In the present study, three Taiwan cobra PLA~2~ variants were prepared by adding an extra __N__‐terminal Met, substituting Asn‐1 by Met or deleting the __N__‐terminal heptapeptide. Recombinant PLA~2~ mutants were expressed in __Escherichia coli__ (__E. coli__), and purified to homogenei

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Modification of His-47 and removal of the N-terminal octapeptide caused a different effect on the structure of Naja naja atra (Taiwan cobra) phospholipase A2 (PLA2). Unlike native enzyme, Ca2+ induced an alteration in the structural flexibility of His-modified PLA2. Moreover, the spatial positions o