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Mutations on the N-terminal region abolish differentially the enzymatic activity, membrane-damaging activity and cytotoxicity of Taiwan cobra phospholipase A2

โœ Scribed by Yi-Ling Chiou; Yun-Ching Cheng; Pei-Hsiu Kao; Jeh-Jeng Wang; Long-Sen Chang


Book ID
116921903
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
511 KB
Volume
51
Category
Article
ISSN
0041-0101

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The structural and functional contributi
โœ Pei-Hsiu Kao; Ku-Chung Chen; Shinne-Ren Lin; Long-Sen Chang ๐Ÿ“‚ Article ๐Ÿ“… 2008 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 384 KB

Modification of His-47 and removal of the N-terminal octapeptide caused a different effect on the structure of Naja naja atra (Taiwan cobra) phospholipase A2 (PLA2). Unlike native enzyme, Ca2+ induced an alteration in the structural flexibility of His-modified PLA2. Moreover, the spatial positions o