Quercetin enhances transforming growth factor β1, secretion by human ovarian cancer cells

✍ Scribed by Giovanni Scambia; Pierluigi Benedetti Panici; F. O. Ranelletti; G. Ferrandina; R. de Vincenzo; M. Piantelli; V. Masciullo; G. Bonanno; G. Isola; S. Mancuso

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: French
• Weight: 594 KB
• Volume: 57
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910570214

✦ Synopsis

Abstract

Our study demonstrates that quercetin (Q)‐induced growth‐inhibitory activity in ovarian cancer cells may be mediated by modulation of transforming growth factor β~1~ (TGFβ~1~) production. We used the OVCA 433 cell line which is very sensitive to the anti‐proliferative effect of Q and expresses high‐affinity, low‐capacity TGFβ~1~ receptors. Conditioned medium (CM) from Q‐treated cells is able to displace ^125I^‐TGFβ~1~ from binding to its receptor; moreover Q (10 μM) increases TGFβ~1~ activity in CM in a time‐dependent fashion starting after 4 hr and reaching a maximum by 24 hr of Q treatment. Q‐induced growth inhibition is reversed by a neutralizing anti‐TGFβ~1~ MAb both in OVCA 433 and in a clonogenic assay of cells from a primary ovarian tumor. Q‐induced increase of TGFβ~1~ activity in CM is specific since other anti‐proliferative compounds, such as Dexamethasone, which is as active on the cell cycle as Q, had no effect on TGFβ~1~ secretion. Northorn‐blot analysis of TGFβ~1~ mRNA levels at various times of Q (10 μM) exposure revealed that there was no increase, suggesting that regulation of TGFβ~1~ occurs at post‐transcription at levels. © 1994 Wiley‐Liss, Inc.