Transforming growth factor-α promotes tumor markers secretion from human ovarian cancers in vitro
✍ Scribed by Hirohisa Kurachi; Hiroshi Adachi; Ken-ichirou Morishige; Kazushige Adachi; Takashi Takeda; Hiroaki Homma; Toshiya Yamamoto; Akira Miyake
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 491 KB
- Volume
- 78
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
BACKGROUND.
The regulatory mechanism of tumor markers secretion has not been well clarified.
METHODS.
Serum levels of CA 125 and tissue polypeptide antigen (TPA) from 17 patients with Stage 111 serous cystadenocarcinoma were measured prior to an initial surgical treatment. Epidermal growth factor receptor (EGFR) status was examined by a n 'LSI-EGF binding assay in a human serous cystadenocarcinoma cell (SHIN-3) and in the 17 primary carcinomas. SHIN-3 cell and the EGFR-expressing primary cancer cells (n = 4) were cultured with or without various concentrations of transforming growth factor (TGF-a), a ligand for EGFR, and the CA 125 and TPA concentrations in the conditioned media were measured.
RESULTS.
EGFR was expressed in 12 primary carcinomas and in the SHIN-3 cell, and it was absent in the remaining 5 carcinomas. Pre-therapeutic serum CA 125 and TPA levels were significantly greater ( P < 0.05) in patients with EGFR-expressing carcinomas (n = 5). These data suggest a possible involvement of EGFR in regulating these tunior markers secretion. TGF-a increased the CA 125 and TPA secretion from SHIN-3 cell. It also promoted the CA 125 secretion in 2 of 4 EGFRexpressing primary ovarian carcinoma specimens.
CONCLUSIONS.
These results suggest that a signal through the EGFR may be involved in regulating the CA 125 and TPA secretion from human ovarian carcinomas.