Quenching of Hoechst 33258 fluorescence in erythroid precursors
β Scribed by Trond Stokke; Harald Holte; Catharina de L. Davies; Harald B. Steen; Sverre O. Lie
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 618 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0196-4763
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## Abstract The bisβbenzimidazole derivative __Hoechst 33258__ (2β²β(4βhydroxyphenyl)β5β(4βmethylpiperazinβ1βyl)β2,5β²βbiβ1__H__βbenzimidazole) binds to the minor groove of DNA duplexes and is widely used as fluorescent cytological stain for DNA. The neutral compound, **1**, is amphiphilic with four
Buoyant density of DNA in CsCl gradients with Hoechst 33258 (bisbenzimide) was investigated as a function of guanine plus cytosine content of the DNA (%GC; in mole percent). A formula for calculating %GC from the refractive index (nD) of the isopycnic CsCl/Hoechst 33258 solution over the range of 0-