A fluorometric procedure is described that can be used in the alkaline elution technique for the measurement of DNA damage in cells whose DNA is not, or cannot be, radioactively labeled. The procedure can be used for the measurement of DNA single-strand breaks, DNA-protein crosslinking, and DNA inte
โฆ LIBER โฆ
EDTA alkaline elution characteristics and measurement of DNA damage in unlabeled DNA using hoechst 33258 fluorescence
โ Scribed by Dr. Jonathan M. Ducore
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 739 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0893-6692
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We have critically evaluated various modifications of the alkaline elution methodology that were required to adapt the method for measuring DNA damage in cells from animal tissues treated in vivo. These modifications involved the use of a fluorometric assay for the eluted DNA using the dye Hoechst 3