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Measurement of DNA damage in unlabeled mammalian cells analyzed by alkaline elution and a fluorometric DNA assay

✍ Scribed by Leonard C. Erickson; Rainhardt Osieka; Nancy A. Sharkey; Kurt W. Kohn

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 538 KB
Volume: 106
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90133-5

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✦ Synopsis

A fluorometric procedure is described that can be used in the alkaline elution technique for the measurement of DNA damage in cells whose DNA is not, or cannot be, radioactively labeled. The procedure can be used for the measurement of DNA single-strand breaks, DNA-protein crosslinking, and DNA interstrand crosslinking, and possibly other DNA lesions produced in unlabeled cells. Although developed for the measurement of DNA damage in tissue-cultured cells, the technique is applicable to the measurement of DNA damage in cells isolated from tissues exposed to DNA damaging agents in vivo.

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