We present a beam-shaping technique for two-photon excitation (TPE) fluorescence microscopy. We show that by inserting a properly designed three-ring pupil filter in the illumination beam of the microscope, the effective optical sectioning capacity of such a system improves so that the point spread
Quasi-isotropic 3-D resolution in two-photon scanning microscopy
✍ Scribed by Ibáñez-López, Cristina; Saavedra, Genaro; Boyer, Gilbert; Martínez-Corral, Manuel
- Book ID
- 115405449
- Publisher
- Optical Society of America
- Year
- 2005
- Tongue
- English
- Weight
- 905 KB
- Volume
- 13
- Category
- Article
- ISSN
- 1094-4087
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