Quantitative uses of affinity chromatography
β Scribed by Irwin M. Chaiken
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 841 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0003-2697
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When using weakly interacting ligands in affinity chromatography, it is possible to take advantage of a true chromatographic process in the separation, as compared with traditional affinity chromatography which is rather an on/off process. In this work, weak monoclonal antibodies were immobilized on
The use of commercially available 5'-UTP-agarose as an affinity chromatography resin for RNase has been described. It was shown that at pH 5.3, 0.025 M piperazine-HCI buffer was effective for the adsorption of active RNase A and exhibited little nonbiospecific binding as has been shown earlier for S