Quantitative semi-automated enzymatic assay for tissue glycogen
โ Scribed by Carr, Robert Scott; Neff, Jerry M.
- Book ID
- 122061287
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 276 KB
- Volume
- 77
- Category
- Article
- ISSN
- 1096-4959
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The physiological role of glycogen phosphorylase is the degradation of glycogen, even though the concentration ratio of glucose-l-P/Pi at equilibrium is ca. 0.28 (equation a) (1) : (glycogen), + Pi = glucose-l-P + (glycogen),-l (a)
cAMP is commonly measured using either immunoassay or high-performance liquid chromatography. The current methods are sensitive but may lack versatility and be expensive; also, radioactivity is potentially harmful to the operator and environment. Given these concerns, we developed a highly sensitive
In the preceding paper (1) we report the development of a new rapid method for the assay of glycogen phosphorylase. During the development of this method a number of conditions were observed to influence the measurement of enzyme activity in the crude extracts. These have been studied systematicall