Quantitative Polymerase Chain Reaction to Estimate the Number of Amplified Esterase Genes in Insecticide-Resistant Mosquitoes

✍ Scribed by M. Weill; C. Berticat; M. Raymond; C. Chevillon

• Book ID: 102564752
• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 58 KB
• Volume: 285
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.2000.4781

✦ Synopsis

ogy as with DSPE-PEG 2000. Since the concentrations of PEG 5000 containing proteins could also be measured by Skoog's method (13), we believe that this modified method would also work.

In conclusion, a simple and direct colorimetric method, modified from Skoog's PEG measurement method (13), for determining the amount of DSPE-PEG 2000 in buffer solutions containing blood or albumin was reported. The concentrations of DSPE-PEG 2000 in those solutions could be determined at 435 nm as low as 25 g/ml with simple sample preparations. It may facilitate the application of PEG-liposomes in drug delivery or blood substitutes.