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Quantitative PCR Analysis of DNA, RNAs, and Proteins in the Same Single Cell

✍ Scribed by Stahlberg, A.; Thomsen, C.; Ruff, D.; Aman, P.

Book ID
115499572
Publisher
American Association for Clinical Chemistry
Year
2012
Tongue
English
Weight
965 KB
Volume
58
Category
Article
ISSN
0009-9147

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## Background Current methods for accurate quantification of nucleic acids typically begin with a template preparation step in which DNA and/or RNA are freed of bound proteins and are then purified. Isolation of RNA is particularly challenging because this molecule is sensitive to elevated temperat