Quantitative measurement of 5-, 12-, and 15-hydroxyeicosatetraenoic acid together with 12-hydroxyheptadecatrienoic acid by stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry

✍ Scribed by H.J. Leis; E. Malle; B. Mayer; G.M. Kostner; H. Esterbauer; H. Gleispach

• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 619 KB
• Volume: 162
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(87)90401-5

✦ Synopsis

A stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry assay for simultaneous quantitative measurement of 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, and 12-hydroxyheptadecatrienoic acid in one single GC/MS run was established. 18O2-Labeled analogs as internal standards, prepared according to conventional procedures, were found to be useful for this application. A sample processing and derivatization sequence providing highly purified compounds with a recovery of 42.7% was elaborated. The detection limit was in the femtomole range.