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Quantitative ion-pair extraction of 4(5)-methylimidazole from caramel colour and its determination by reversed-phase ion-pair liquid chromatography

โœ Scribed by Mohens Thomsen; Dorthe Willumsen

Book ID
108334851
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
559 KB
Volume
211
Category
Article
ISSN
1873-3778

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A new method to determine the guanosine 5',3'-bisdiphosphate (ppGpp) concentration in bacterial cultures has been developed. Cells in which the nucleotide pool was stabilized by treatment with formaldehyde were concentrated from samples of cultures and lysed by alkali. The ppGpp was separated from o