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Improved method for determination of 5-methylcytosine by ion-pair reversed-phase high-performance liquid chromatography

โœ Scribed by L. Citti; P.G. Gervasi; G. Turchi; L. Mariani; M. Durante

Book ID
108336781
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
289 KB
Volume
261
Category
Article
ISSN
1873-3778

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Quantitative determination of 5-methylcy
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โœ Dirk Eick; Hans-Joachim Fritz; Walter Doerfler ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 518 KB

A method to separate the four major bases (cytosine, guanine, thymine and adenine) and the two minor modified bases (5-methylcytosine and 6N-methyladenine) in DNA has been developed. For optimal separation, several different buffer systems are available for isocratic elution. The 12 5-methylcytosine