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Quantitative in situ hybridization: Expression of the type II collagen gene in growth cartilage

✍ Scribed by Assem, Kak; Dominique, Le Guellec; Damien, Schoëvaërt; Nicole, Balmain

Book ID
122334160
Publisher
Portland Press
Year
1992
Tongue
English
Weight
124 KB
Volume
76
Category
Article
ISSN
0248-4900

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✍ Ei-ichi Takahashi; Tada-aki Hori; Peter O'Connell; Mark Leppert; Ray White 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 714 KB

A new mapping system, based on nonisotopic in situ hybridization combined with fluorescent staining of replicated prometaphase R-bands, is described. Replication of the bands is achieved by treatment of thymidine-synchronized cells with bromodeoxyuridine. The human COL2A1 gene was mapped to band 12q