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Quantitative in situ gel electrophoretic assay for neomycin phosphotransferase activity in mammalian cell lysates

โœ Scribed by Nevis Fregien; Norman Davidson


Book ID
107714246
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
700 KB
Volume
148
Category
Article
ISSN
0003-2697

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A dot assay for determining neomycin phosphotransferase (NPT II) activity in crude cell extracts has been developed. The assay provides for the rapid screening of large numbers of cell cultures generated in gene transformation experiments using NPT II as a dominant selectable marker. Currently, the