A quantitative assay for neomycin phosphotransferase activity in plants
โ Scribed by Mark Staebell; Dwight Tomes; Arthur Weissinger; Sheila Maddock; Wallace Marsh; Gary Huffman; Ronald Bauer; Margit Ross; John Howard
- Book ID
- 107714871
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 603 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0003-2697
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A simple method for the measurement of neomycin phosphotransferase (NPT) activity in crude extracts of eukaryotic cells is described. This method is based on the elimination of interfering phosphorylated proteins by using phenol-chloroform extraction. This solution phase assay allows the detection o
A dot assay for determining neomycin phosphotransferase (NPT II) activity in crude cell extracts has been developed. The assay provides for the rapid screening of large numbers of cell cultures generated in gene transformation experiments using NPT II as a dominant selectable marker. Currently, the