## Recent advances in reporter gene technologies are now allowing us to image gene transcription at the single cell level, using either fluorescence or luminescence microscopy. Here, the basis of these techniques is outlined and their advantages and disadvantages in various biological systems are
β¦ LIBER β¦
Quantitative Imaging of Protein Secretions from Single Cells in Real Time
β Scribed by Raphael, Marc; Christodoulides, Joseph; Delehanty, James; Long, James; Byers, Jeff
- Book ID
- 122996009
- Publisher
- Biophysical Society
- Year
- 2014
- Tongue
- English
- Weight
- 62 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0006-3495
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## Abstract We have developed a new in vivo mouse model to image single cancerβcell dynamics of metastasis to the lung in realβtime. Regulating airflow volume with a novel endotracheal intubation method enabled controlling lung expansion adequate for imaging of the exposed lung surface. Cancer cell