Quantitative GLC determination of conjugated estrogens in raw materials and finished dosage forms

✍ Scribed by R. Johnson; R. Masserano; R. Haring; B. Kho; G. Schilling

• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 563 KB
• Volume: 64
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600640626

✦ Synopsis

A GLC method is described for the quantitative analysis of conjugated estrogens. The procedure was used for the analysis of various estrogen mixtures obtained from formulations containing estrogen sodium sulfate salts. The method involves slurrying or dissolving a portion of the formulation in p H 5.2 buffer, hydrolysis with sulfatase enzyme, extraction with ethylene dichloride, and, finally, GLC separation and quantitation of the corresponding trimethylsilyl ethers on a 2.5% diethylene glycol succinate column.

Keyphrases Estrogen sodium sulfate salt formulations-enzymatic hydrolysis, GLC separation and analysis of conjugated estrogens Conjugated estrogen mixtures-enzymatic hydrolysis of estrogen sodium sulfate salt formulations, GLC separation and analysis GLC-separation, analysis, conjugated estrogens in estrogen sodium sulfate salt formulations