Quantitative gas-liquid chromatographic determination of ftorafur and 5-fluorouracil in biological specimens

✍ Scribed by C. Pantarotto; R. Fanelli; S. Filippeschi; T. Facchinetti; F. Spreafico; M. Salmona

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 413 KB
• Volume: 97
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90350-6

✦ Synopsis

A method developed for measuring the antitumor agent ftorafur and its biotransformation product Muorouracil was applied to biological specimens. After extraction with ethylacetate, ftorafur, S-fluorouracil, and the internal standard 2-methyl-4-hydroxy-6-chloromethylpyrimidine are converted to their chloromethyldimethylsilyl derivatives and assayed by glc, using either an electron-capture or a flame ionization detector. The minimum detectable amount is 100 pg/injection for ftorafur and 50 pg/injection for 5fluorouracil employing electron-capture detection. Linearity was found up to microgram amounts of both substances, without any interference from endogenous substrates. Preliminary data are reported on the comparative serum kinetics of ftorafur and 5-fluorouracil in mice.