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Quantitative extraction and estimation of intracellular nucleoside triphosphates of Escherichia coli

โœ Scribed by Aldo S. Bagnara; Lloyd R. Finch

Book ID
107711265
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
715 KB
Volume
45
Category
Article
ISSN
0003-2697

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Quantitative extraction and estimation o
Quantitative extraction and estimation of intracellular nicotinamide nucleotides of Escherichia coli
โœ Esa-Matti Lilius; Veli-Matti Multanen; Veija Toivonen ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 433 KB

The conditions of extraction of nicotinamide nucleotides from Escherichia coli were systematically investigated. The optimum pH for NAD+ and NADP+ appeared to be around 1.5 while that for NADH and NADPH was around 12.5. Seven minutes at 60ยฐC was chosen for the extraction of all four nucleotides. Add