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Quantitative determination of the main glucose metabolic fluxes in human erythrocytes by 13C- and 1H-MR spectroscopy

✍ Scribed by Irene Messana; Francesco Misiti; Said el-Sherbini; Bruno Giardina; Massimo Castagnola


Book ID
117331539
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
344 KB
Volume
39
Category
Article
ISSN
0165-022X

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## Abstract A simple and sensitive NMR method for quantifying excess ^13^C‐enrichment in positions 2 and 3 of lactate by ^1^H NMR spectroscopy of the lactate methyl signal is described. The measurement requires neither signal calibrations nor the addition of a standard and accounts for natural abun